HPLC Tips

HPLC Tips – High Performance Liquid Chromatography Troubleshooting

The tips and tricks here are intended as a quick reference to solve common but tricky HPLC problems that can slow or stop laboratory throughput. If there is a hplc problem you need solved that is not listed here, submit details to SC Chromatography and we will get you the solution. SC Chromatography provides no warranty for the HPLC tips and tricks presented here. HPLC problems or issues that cannot be addressed here may require an onsite service call by an engineer. Please call 310-538-4331 to get in touch with our HPLC engineers.

Leaks
Leaks are the easiest problems to
Pressure Problems
Pressure problems are observed as a change in the system pressure. Standard operating pressure for a given assay or system condition should be recorded and used as a reference in order to determine when a problem with system pressure exists. High pressure may result from changes in system components (column length, diameter or particle size), an inadvertent change in flow rate, change in mobile phase composition, or particulate buildup within the system.
Be sure to check the column specifications…
Retention-Time Problems
Before beginning HPLC troubleshooting procedures related to retention time changes, compare the retention times of several runs to verify that the problem is reproducible and determine if there is a pattern to the variation.

Variable retention time
A shift of all peaks
When retention time changes for selected peaks
Thorough column equilibration
HPLC column problems
HPLC column temperature problems
The sample and injection solvent
Insufficient sample preparation

HPLC Problems or issues that cannot be addressed here may require an onsite service call by a HPLC- High Performance Liquid Chromatography Engineer.

For Help and information on HPLC technology please contact us at 310-538-4331 to get in touch with our engineers. Please keep checking in for more HPLC Tips and Tricks.

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